Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.5, 500 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.5
Alkali Scientific HEPES Buffered Saline Solution 2X, 500 Milliliters
HEPES Buffered Saline Solution 2X is a sterile, concentrated buffer solution used in biological and biochemical applications where stable pH control is required. The 2X concentration provides a convenient way to dilute the solution to the desired pH range for specific experiments, such as protein analysis, enzyme assays, and cell culture maintenance. HEPES is a widely used buffer in cell and molecular biology due to its minimal interference with biological processes and its ability to maintain a stable pH in a variety of biological environments. The 500mL bottle size is ideal for routine laboratory use in large-scale research and clinical applications.
Biotang Inc TRIS-HEPES-SDS Running Buffer (10X), 500 ml
TRIS-HEPES-SDS Running Buffer (10X), 500 ml.
Bioworld PEB- Protein Extraction Buffer (5x), 500 mL
Tris Protein Extraction Buffer (PEB), 5X Lysis buffer for extracting proteins from cells. Technical Information:5X concentrationTris-HCL, pH 8.0 (0.5M)B-mercaptoethanol (100mM)EDTA (20mM)Add protease inhibitor according to your protocol
Teknova 1M Tris-HCl, pH 7.5 Solution, 20L Bag, Sterile. 4 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Sigma Aldrich Fine Chemicals Biosciences TRIS 3 HYDROXYPROPYLTRIAZOLY1G
Tris(3-hydroxypropyltriazolylmethyl)amine, 1g
![eMolecules 1446012-48-1 | TERT-BUTYL 6-(HYDROXYMETHYL)-5-OXA-2-AZASPIRO[3.4]OCTANE-2-CARBOXYLATE | MFCD27986992 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502181707.JPG-250.jpg)
eMolecules 1446012-48-1 | TERT-BUTYL 6-(HYDROXYMETHYL)-5-OXA-2-AZASPIRO[3.4]OCTANE-2-CARBOXYLATE | MFCD27986992 | 1g
Medchem Express | Deschloroclozapine | 10mg | 446270896 | HY-42110 | 1977-07-7 | MFCD01730958 | 292.386 | C18H20N4
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S2 Media 1L 10x TRIS Buffered Saline (TBS, 10x), pH 7.4 in 1L Media Bottle, 1/each
S2Media™ 10x TRIS Buffered Saline (TBS, 10x), pH 7.4 is a ready to use stock buffer often used for immunodetection techniques, including Western blot protocols. To prepare a 1x solution, mix one volume of 10x solution with 9 volumes of 10x buffer solution.• Product meets reference methods and performance criteria.• TBS,10x should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions. • Product is 0.1µm filtered. Bottles are RNase, DNase, and protease free.
Boston Bioproducts Inc Sodium Phosphate Monobasic (3 M) - 2x1L
Bioprocess Grade - Sodium Phosphate Monobasic (3 M) - 2x1L (For Research Use and Further Manufacturing Only)
eMolecules 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 20656-08-0 | MFCD00141691 | 1g
Matrix Scientific | 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 1g | 389721058 | 45569 | 95.000 | 20656-08-0 | MFCD00141691 | 234.080 | C9H9Cl2NO2
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Medchemexpress LLC Mops (sodium salt) | 71119-22-7 | 99.83% | 10 G
The product is a buffering agent utilized in biology. It serves to maintain the pH of mammalian cell culture media and notably enhances the thermal stability of BSA.
- Functions as a buffering agent in biological applications.
- Maintains the pH level of mammalian cell culture media.
- Significantly improves the thermal stability of BSA.
- Can be used in the preparation of keratinocyte and microbial culture medium.
- Relatively safe for short-term experiments, though prolonged use may reduce cell viability.