Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
LAB BIOREAGENTS LowBinding IV Buffer 250ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
Alkali Scientific CellPro™ TAE, 10X (Tris acetate EDTA), 4L Cubitainer
Alkali Scientific™ CellPro™ TAE Tris Acetate EDTA is a fundamental reagent in nucleic acid electrophoresis, renowned for its exceptional buffering capacity and DNA solubilization properties. Designed to provide optimal performance and reproducibility, this solution enables precise separation and analysis of DNA fragments in agarose gel electrophoresis. Each batch undergoes rigorous sterile filtration to ensure purity and consistency, empowering researchers with confidence in their molecular biology experiments.
Electron Microscopy Sciences Tris Buffer 0.2M, pH 8.0 250 ML
C₄H₁₁NO₃ F.W. 121.14
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged.
pH: 8.0
Medchemexpress LLC NVP-TAE684 | 761439-42-3 | MFCD11977634 | 99.7% | 614.2 g/mol | C30H40ClN7O3S | 10 MG
NVP-TAE684 is a potent, selective small-molecule inhibitor of anaplastic lymphoma kinase (ALK) used in biochemical and cellular research. It blocks growth of ALK-dependent and ALCL-derived cell lines with low-nanomolar IC50 values and has been applied in preclinical studies to probe ALK signaling and validate target engagement. Typically supplied as a solid for in vitro experiments.
- Potent ALK inhibition at low-nanomolar concentrations.
- Selective activity against ALK-driven cell lines.
- Suitable for biochemical and cellular assays.
- Supplied as a solid for research use.
- Characterized with reported purity and analytical data.
Alkali Scientific CellPro™ Tris-HCL Buffer,1M, pH 8.0, 1L, 12/cs
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | 1185-53-1 | MFCD00012590 | 100G
Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 157.6 | 1185-53-1 | MFCD00012590 | 100G
![Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 250 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60050-250.0.JPG-250.jpg)
Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 250 Grams
TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.
It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques.
TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis.
One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.
Rockland Immunochemicals 1X TES pH 7.8 (10 mM Tris HCl, 100 mM NaCl, 1 mM EDTA) DEPC TREATED - MB-058
1X TES pH 7.8 DEPC TREATED - TES buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. TES buffer is useful to stabilize proteins, DNA, or RNA, while protecting it from degradation.